Figure 2

Localization of dhfr and other genes in chromosome 5 that are overexpressed in HT29 MTX-resistant cells. It is presented a magnification of the region in chromosome 5 where dhfr is located (5q14). The left part is an ideogram of chromosome 5; the right part shows the relative position of all genes studied that are located in this chromosome and that were amplified. The arrows indicate their transcription orientation and the values in parentheses under the names correspond to their respective copy-number validated by Real-time PCR.