Figure 3From: Role of Caveolin 1, E-Cadherin, Enolase 2 and PKCalpha on resistance to methotrexate in human HT29 colon cancer cells Determination of mRNA levels and cytotoxicity upon siRNA treatment of HT29 sensitive cells. (A) The mRNA levels of CAV1, ENO2, PKCα and DHFR were determined by RT-Real-time PCR 48 h after treatment of HT29 cells with the indicated concentrations of siCAV1, siENO2, siPKCα, siDHFR and a non-related siRNA. Symbols used for each mRNA are presented as an insert within the figure. (B) Cells were treated with 100 nM of each siRNA as previously described and 5 × 10-8 M MTX was added after 48 h. Cell viability was determined after 5 days from the beginning of the treatment. All results are expressed as percentages referred to untreated cells. Values are the mean of three independent experiments ± SE. A non-related (NR) siRNA was used as negative control.Back to article page