Treatment | Cell line | CAV1 | E-cadherin |
---|
siCAV1 + pBATEM2-CDH | HT29 sensitive | 21.8 ± 1.9 | 252.3 ± 3.5 |
siCAV1 + pBATEM2-CDH | HT29 resistant | 25.6 ± 0.2 | 199.7 ± 16.9 |
- Transfection experiments combining the siRNA against caveolin 1 (siCAV1) and the expression plasmid for E-cadherin (pBATEM2-CDH) were performed both in HT29 sensitive and MTX-resistant cells. Forty-eight hours later, mRNA levels for the two genes were determined in both cell lines by RT-Real-Time RCR. Results are expressed as percentages of mRNA referred to untreated cells (mean ± SE) of at least 3 independent experiments.