Figure 1

Expression profiles of genes associated with regulation of apoptosis. Genes that were differentially expressed temporally in T-cell activation of the three (CD3+, CD4+ and CD8+) populations were divided into two groups (A with mostly upregulated genes, and B with mostly downregulated genes) according to their distinct expression patterns based on hierarchical clustering using the Euclidean distance metric. Color denotes degree of differential expression compared to 0 hour (saturated red = 3-fold up-regulation, saturated green = 3-fold down-regulation, black = unchanged, gray = no data available). Expression data shown are averages from three independent biological experiments for each T-cell population. The median ratio, along with the maximum (for up-regulated genes) or minimum (for down-regulated genes) ratio of stimulated T cells at each timepoint (with respect to the expression of 0 hour) is provided (a negative value represents down-regulation). Pro-apoptotic genes names and descriptions are shown in red, anti-apoptotic genes are shown in green and genes with both pro- and anti-apoptosis roles are shown in blue. Genes with unknown functions in apoptosis are shown in black. (C) Schematic view of significantly regulated genes of BCL2 family. Green and red connections denote negative and positive regulation of apoptosis, respectively, based on information of NCBI website [18]. Regulation of gene transcription in CD3+ T cells, compared to 0 hour, is denoted by different color (green: downregulation, red: upregulation) at each timepoint in the sequence of 4, 10, 48 and 96 hours.