Table 1 Primers for real time PCR, amplicon size, values of slope, PCR efficiency and linearity (R2).
Genes | Primers | 5' → 3' | Amplicon size (bp) | Slope | PCR Efficiency | R2 |
|---|---|---|---|---|---|---|
GNA15 | Forward | GAGAACCGCATGAAGGAGAG | 84 | -3.312 | 100.0 | 0.991 |
| Â | Reverse | AAAGAGGATGACGGATGTGC | Â | Â | Â | Â |
KRT31 | Forward | TGAGCAGGAGGTCAATACCC | 110 | -2.913 | 120.4 | 0.990 |
| Â | Reverse | GACTCCTGGTCTCGTTCAGC | Â | Â | Â | Â |
BST2 | Forward | GGAGGAGCTTGAGGGAGAG | 75 | -3.476 | 93.9 | 0.991 |
| Â | Reverse | CTCAGTCGCTCCACCTCTG | Â | Â | Â | Â |
MFAP2 | Forward | GCCGTGAGGAACAGTACCC | 91 | -3.152 | 107.6 | 0.990 |
| Â | Reverse | CGGAGGCTGTAGAAGCAGAC | Â | Â | Â | Â |
TUBA6 | Forward | TCAACACCTTCTTCAGTGAAACG | 101 | -3.341 | 99.2 | 0.991 |
| Â | Reverse | AGTGCCAGTGCGAACTTCATC | Â | Â | Â | Â |
GAPDH | Forward | ACCCACTCCTCCACCTTTGA | 101 | -3.392 | 97.1 | 0.990 |
| Â | Reverse | CTGTTGCTGTAGCCAAATTCGT | Â | Â | Â | Â |
ACTB | Forward | GGCACCCAGCACAATGAAG | 66 | -3.255 | 102.8 | 0.994 |
| Â | Reverse | CCGATCCACACGGAGTACTTG | Â | Â | Â | Â |