Figure 8

qPCR time-dependent validation of core HDACi-repressed gene expression changes in HCT116 and HT29 cells. HCT116 and HT29 cells were treated with 2 μM vorinostat (Vor) or 50 nM LBH589 for 6, 12 and 24 h. Total RNA was extracted, reverse transcribed and qPCR analysis was performed as described in the 'materials and methods' using the primer sets given in Table 4. Histogram bars represent the mean ± SD for two independent RNA isolations analyzed in triplicate. All genes were normalized to GAPDH, * denotes p-value < 0.05 for both HDACi treatment groups when compared to respective time-matched control.