Association of adipocyte genes with ASP expression: a microarray analysis of subcutaneous and omental adipose tissue in morbidly obese subjects

Table 2 Real Time PCR Analysis of Selected Genes in Subcutaneous and Omental LAT and HAT Groups

Gene	Accession #	Subcutaneous			Omental
		LAT	HAT	%	LAT	HAT	%
C5L2	NM_018485	0.950 ± 0.105	1.958 ± 0.237**	206% (NA)	1.161 ± 0.096	2.684 ± 0.263**	231% (NA)
C3	NM_000064	0.588 ± 0.086	0.928 ± 0.117*	158% (134%)	2.655 ± 0.829	2.344 ± 0.026	88% (45%)
C5aR	NM_00173	1.412 ± 0.189	1.588 ± 0.120	112% (78%)	1.738 ± 0.240	1.926 ± 0.229	111% (122%)
CD36	NM_000072	0.877 ± 0.134	1.717 ± 0.212**	196% (268%)	0.411 ± 0.072	0.874 ± 0.103**	213% (96%)
DGAT2	NM_032564	1.671 ± 0.358	3.383 ± 0.513*	202% (245%)	0.606 ± 0.207	1.110 ± 0.151*	183% (144%)
FABP4	NM_001442	0.732 ± 0.093	0.776 ± 0.075	106% (128%)	0.638 ± 0.064	0.739 ± 0.083	116% (92%)
CEBPγ	NM_001806	1.006 ± 0.226	1.497 ± 0.126*	160% (143%)	3.371 ± 1.694	3.607 ± 1.318	97% (75%)
INSR	X02160	1.553 ± 0.278	1.580 ± 0.252	102% (112%)	1.407 ± 0.617	1.630 ± 0.371	116% (62%)

Gene expression in SC and OM adipose tissue from Lower ASP and TG (LAT) and Higher ASP and TG (HAT) subjects as assessed by ΔΔ real time RT-PCR relative to the housekeeping gene B2M. Data are means ± SEM (n = 8 to 16 total per group) and are analysed by t test, where * p < 0.05, and ** p < 0.01. Percentage change of HAT vs LAT is provided, with the comparative value from microarray analysis (see Figures and Additional File 1, Table S1 for data) given in parentheses, where NA = not available.

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