Figure 3From: Concomitant heterochromatinisation and down-regulation of gene expression unveils epigenetic silencing of RELBin an aggressive subset of chronic lymphocytic leukemia in males Distribution of HP1α, 3metH3K9 proteins and 5metC in human CLL cells. Representative images are shown for the localization of HP1α (a-x). All cells were stained with DAPI (blue) (a,e,i,m,q,u). Cells were stained with a polyclonal antibody against 3metH3K9 (red) (b,f,j,n,r,v) a marker of inactive chromatin and antibody against HP1α (green) (c,g,k,o,s,w) a marker of heterochromatin. Merged images show the co-localization of these two proteins. Labeling with a 5metC antibody (green) (1-6) revealed a global hypomethylation state and a more diffuse pattern in CLL cells (1-4) vs. control cells (5-6). Scale bar, 2 μm.Back to article page