Figure 1From: Integrative analysis of next generation sequencing for small non-coding RNAs and transcriptional regulation in Myelodysplastic Syndromes NGS data analysis pipeline and comparison of sRNA annotations in MDS. NGS data analysis pipeline used for this study. In A) we show the annotation of a sequence read. It was detected about 18000 times in RAEB2 and aligned at nine different positions, spread over six chromosomes, on the human genome (green). A single alignment position is shown (red) with the used annotation hierarchy (blue). The purple callbox, details the matched loci for miRNA let-7a-1, its full primary sequence (top), its mature sequence (middle) and the aligned short read (bottom). The brown callbox shows all nine annotations, including a number of miRNAs from the has-let-7 family as well as a piRNA. In B) we compare the total RNA content measured from our high-throughput sequencing and annotation steps, on the left results for the RAEB2, in the middle results for RA and on the right results for control.Back to article page