Figure 3From: Molecular diagnostics for congenital hearing loss including 15 deafness genes using a next generation sequencing platform Workflow for the PCR enrichment approach. Amplicons are equimolar pooled per patient, continuously the A and B (454) adapters with MID are ligated to each pool of amplicons. All the patients, each tagged with a unique MID, can be pooled before proceeding to emulsion PCR.Back to article page