Figure 4

miRNA down-regulation in bladder cell lines. LNA based antagomirs were used to silence miR-27a, miR-138, miR-296-5p, miR-642 and miR-886-3p in the bladder cell lines 253JBV, UMUC9, UMUC14, SLT4, 575A, RT4, CLR2169 and HT1197. LNA knockdown molecules were reverse transfected at 20nM (n = 8), and after 48 h incubation culture media, with or without cisplatin (GI50), was added to the cells (n = 4). The viability of the cells was determined after 48 h incubation (96 h post transfection) by MTT-assay. LF2000 designate controls using the transfection reagent alone. A: Relative cell viability expressed as the viability of the antagomiR transfected cells normalized to the negative control molecule for each cell line. B: Relative cell viability expressed as the viability of the antagomiR transfected cells treated with cisplatin (GI50) compared to the antagomiR transfected cells without cisplatin normalized to the negative control molecule for each cell line. (#: the high reduction of cell viability imposed by the antagomirs prohibits precise measure of effect of cisplatin treatment during miR down regulation).