Fig. 1

a Transcriptome analysis of RNAs isolated from AF at different gestational stages. RNAs isolated from 16 AF samples (4 Prenatal: 18-24 weeks, 6 Late Preterm: 34–36 weeks, 6 Term:39–40 weeks) were subjected to the AF transcriptome analysis. b Principal component analysis (PCA) of the top 3 identified components of RNA-seq samples showing the primary separation of samples by gestational ages. Prenatal (Green), Late Preterm (Blue) and Term (Red). c Heatmap produced by hierarchical clustering of genes and samples, showing the identification of differentially expressed genes in prenatal vs later preterm vs term AF samples