Gene and protein analysis reveals that p53 pathway is functionally inactivated in cytogenetically normal Acute Myeloid Leukemia and Acute Promyelocytic Leukemia

Table 3 Comparison of our bioinformatics results to literature-based gene expression signatures of DNA damage-induced response

Ref	System	Literature-based gene signature		Our bionformatic results
		Number of genes altered in the signature	Number of genes included in our p53 list	DEGs in CN-AML	DEGs in APL
[85]	p53-dependent transcriptional response of cell lines to 9 DNA-damaging agents	16 ↑	12↑	0 ↑ 1 ↓	0↑ 1 ↓
[86]	transcriptional response of human cells to ionizing radiation	199 ↑ 49 ↓	59 ↑ 11 ↓	1↑ 6↓	1↑ 7↓
[87]	healthy PB cells irradiated ex vivo	61^a	29	3	5
[88]	34 patients before and after irradiation^b	23^a	12	1	1
[89]	7 AML patients before and after chemotherapy^c	30↑ [113 ↑]^d	29↑	1↑ 2↓	0↑ 3↓
	Total		152	33/152 DEGs, 119/152 not DEGs

We compare how many of upregulated/downregulated genes in the signature are indeed upregulated or downregulated DEGs in our analysis of CN-AML and APL, ↑- upregulated gene expression, ↓- downregulated gene expression
^aOnly the list of the signature genes was provided, without the specific names of up- and down-regulated genes. Therefore, we present the overall number of upregulated and downregulated DEGs
^bAll are hematological patients
^c5 are CN-AML patients and 1 is APL
^d30 of the upregulated 113 genes are associated with p53 regulation

ISSN: 1755-8794