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Table 1 The sequence of the primers and PCR–RFLP products characteristics

From: Vitamin D receptor rs3782905 and vitamin D binding protein rs7041 polymorphisms are associated with hepatocellular carcinoma susceptibility in cirrhotic HCV patients

SNPS

Primer Sequance

Annealing Temprature

Restriction enzyme

Product Size

rs2228570

F: 5'-AGCTGGCCCTGGCACTGACTCTGGCTCT-3'

CËš60

BseG I

CC: 267 bp

 

R: 5'-ATGGAAACACCTTGCTTCTTCTCCCTC-3'

  

CT: 267 + 204 + 63 bp

TT: 204 + 63 bp

rs3782905

F: 5'-AAGACATGGTGTCTGCTTCA-3'

56.0ËšC

HpyF3 I

CC: 223 + 81 bp

CG: 304 + 223 + 81 bp

 

R: 5'-GGTTAGATCGATATGTTTGA-3'

  

GG: 304 bp

rs7041

F: 5'-AAATAATGAGCAAATGAAAGAAGAC-3'

56.0ËšC

BsuR I

TT: 482 bp

TG: 482 + 298 + 184 bp

 

R: 5'-CAATAACAGCAAAGAAATGAGTAGA-3'

  

GG: 298 + 184 bp

rs11568820

F1: 5'-AGGATAGAGAAAATAATAGAAAACATT-3'

45.0ËšC

 

GG: 297 + 110 bp

 

R1: 5'-AACCCATAATAAGAAATAAGTTTTTAC-3'

  

GA: 297 + 235 + 110 bp

 

F2: 5'-TCCTGAGTAAACTAGGTCACAA-3'

  

AA: 297 + 235 bp

 

R2: 5'-ACGTTAAGTTCAGAAAGATTAATTC-3'

   
  1. Enzyme (BseG I) investigates SNP (rs2228570), Enzyme (HpyF3 I) investigates SNP (rs3782905), Enzyme( BsuR I) investigates SNP (rs7041)