Fig. 6

Volcano plots and heatmaps of DEGs (left) and DMRs (right) by variants in TCGA-COAD. The VX means a patient who does not have variants (n = 429 in DEGs, n = 269 in DMRs) and the VY (n = 21 in DEGs, n = 20 in DMRs) is the opposite. a Volcano plots indicate upregulated (red dots) and downregulated (green dots) DEGs and DMRs in TCGA-COAD RNA-seq data and Illumina 450 k chip analysis in each. Two axes indicate P-value and fold change between two groups which has variants.Ten DEGs meet criteria |FC| > 0.2 and p-value < 0.01. Eight DMRs also meet criteria |FC| > 0.2 and p-value < 10⁻¹². The dashed green and red lines indicate where |FC| > 0.2, and the dashed blue line means the criteria of p-values. b Heatmaps of DEGs and DMRs representing differences between vital status, gender, and presence of variants. Vital status, gender, and presence of variants are indicated as column annotation bars. Other annotation bars indicate fold change and p-value between the two groups