Fig. 4

Comparative analysis of the stability between TMC1 wild-type, p.Phe419Ser and p.Trp482Arg proteins using CHX chase assays in a transient overexpression system. HEK293T cells overexpressing TMC1 were treated with CHX at a concentration of 80 µg/ml for a duration of up to 3 h to inhibit general translation. In the immunoblots, the observed two bands for TMC1 wild-type and mutants might represent phosphorylated TMC1 [9]. The CHX chase assay was conducted once, with three measurements acquired throughout the experiment. Consequently, each experimental condition had a sample size of three. The original blots were represented in Additional file 4: Fig. S3