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Fig. 4 | BMC Medical Genomics

Fig. 4

From: Novel compound heterozygous MYO15A splicing variants in autosomal recessive non-syndromic hearing loss

Fig. 4

Minigene splicing assay of c.6177 + 1G > T and c.9690 + 1G > A in MYO15A using pET01 vector. A The RT-PCR results of minigene splicing assay are shown. M: DNA marker; 1–5 represent RT-PCR products of HEK293 cells transfected with the following five plasmids: (1) pET01-Ex28-WT plasmid; (2) pET01-Ex28-MT plasmid; (3) pET01-Ex59-WT plasmid; (4) pET01-Ex59-MT plasmid; (5) empty pET01 vector; (6) no template negative control. B Graphical illustration of the expected splicing products. C Representative Sanger sequencing results of the splicing products in HEK293 cells transfected with different plasmids

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BMC Medical Genomics

ISSN: 1755-8794

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