Table 1 Primer sequences for the long-range PCR of the four common deafness genes
Gene | Forward | Primer sequence | Reverse | Primer sequence |
|---|---|---|---|---|
MT-RNR1 | 12SrRNA-F | GTTTAGACGGGCTCACATCACC | 12SrRNA-R | GGTTTGGCTAAGGTTGTCTGGT |
GJB2 | GJB2-F | ATGTCCAGCCAACTCAGCATTCAC | GJB2-R | ACAAGCCGACTCAGCACATCTCTC |
GJB3 | GJB3-F | GGCTCCCAACTCAGTGCTCATTCTC | GJB3-R | TGATGACCCCACCTGTCTTTGCTG |
SLC26A4 | SLC26A4-1 F | AAATCGTGAACTCTCTGTATCCTCC | SLC26A4-1R | GCTGACCTCAAACATCTACCTTATC |
SLC26A4 | SLC26A4-2 F | CAGATGTAGATGCCTTCAGTCCAGT | SLC26A4-2R | TCCAGAGCAGCCTTTACTTCATTAG |
SLC26A4 | SLC26A4-3 F | TCTAATGAAGTAAAGGCTGCTCTGG | SLC26A4-3R | CGGCTGTTTTCATTATCCTTGTCT |
SLC26A4 | SLC26A4-4 F | AGGTCCTTCATTCATCTCTGTCAC | SLC26A4-4R | GGATTTCAGTTCAGGCACTTAGAC |
SLC26A4 | SLC26A4-5 F | ATGTCCATTCTCCTGATGTTACCTC | SLC26A4-5R | TGGGCAACCCTACCTCCAATAC |
SLC26A4 | SLC26A4-6 F | TTACTGGGGTCCAATCAGGAATAG | SLC26A4-6R | GCTTTGCTCTCCTCATAACAACATC |
SLC26A4 | SLC26A4-7 F | ATGGAAATGTGTATCTTGACAGTGG | SLC26A4-7R | GCCTCACAAGAATGTTCATAGTAGC |