Fig. 3From: A novel de novo CLTC variant altering RNA splicing causes fetal developmental abnormalitiesMinigene assay revealed the CLTC variant c.3249 + 1G > C altered the RNA splicing. A Agarose gel electrophoresis result showing that the mRNA of the MT was longer than the WT. B Sanger sequencing of the RT-PCR product showed the WT was 594Â bp and MT was 762Â bp with the retention of intron 20. C Schematic diagram showing the aberrant splicingBack to article page