Fig. 8

Results Validation in GSE35958 dataset. A GO enrichment results of differentially expressed iron-metabolism-related genes. The outmost side of the left circle is the GO term ID. The middle ring represents upregulated iron-metabolism-related genes. Each dot represents one gene that is enriched in this GO term. The color of the innermost circle represents the z-score. A gene that is closer to red has a greater upregulation degree. The length of the color bar represents the adjusted P-value. A longer color bar means a smaller adjusted p-value. B KEGG enrichment results of differentially expressed iron-metabolism-related genes. The dot size represents a number of genes in the pathway. The pink color bar represents fold change value of genes. The number of dots means gene entrez ID. C Heatmap of the Pearson correlation between immune cell infiltration levels through CIBERSORT and differentially expressed iron metabolism-related genes. Red represents negative correlation and blue represents positive correlation. D Differences in immune infiltration between osteoporosis and healthy group. Y-axis indicates the CIBERSORT immune infiltration results, and the x-axis indicates different immune cells. Yellow presents the osteoporosis group (OP), and green presents normal control (NC). * means p < 0.05, ** means p = 0.01, *** means p = 0.001, **** means p = 0.0001, ns or blank means no significant difference. E Protein-protein interaction (PPI) network of differentially expressed iron metabolism-related genes. The colors changing from yellow to green represent an increase in the degree of nodes. F Top five hub genes. The color of the dots represents the MCC score, and the color changing from yellow to red indicates a gradually increasing score. The size of the dots indicates the size of the degree. G The hub-drug sub-network. Orange dots represent hub nodes, blue triangles represent drugs, and the dots’ size indicates the degree’s size. H The hub-TF network. Orange dots represent hub nodes, yellowish-green squares represent TFs, and the dots’ size indicates the degree’s size. I The hub-miRNA sub-network. Orange dots represent hub nodes, green arrows represent miRNAs, and the dots’ size represents the degree’s size