Fig. 9

Expression analysis of NUPR1 at transcription and translation Levels. Representative images (A) and quantification (B) of NUPR1 in intratumoral and peritumoral fractions through immunohistochemistry staining (N = 6). MRNA (C) and protein expression (D&E) of NUPR1 in cell lines (N = 3). (F) Cell viability assessed through CCK8 assays between saline and trifluoperazine subgroups (N = 6). (G) Representative images and results of cell counting from the Transwell invasion assay (N = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001