From: A novel COL4A5 splicing mutation causes alport syndrome in a Chinese family
Name | Primers | Sequence (5′-3′) | Product length |
---|---|---|---|
Round 1 amplification primers for Nested PCR | 245,465-COL4A5-F | ctaactggtgtctagctggc | 3274 bp |
248,738-COL4A5-R | gaatggcccagacaatgaga | ||
Round 2 amplification primers for Nested PCR | 245,699-COL4A5-F | catcccagaagcaaatggat | 2734 bp |
248,432-COL4A5-R | aggctgaggcagaagaattg | ||
pcMINI-N-wt/mut full-length amplification | pcMINI-N-COL4A5-HindIII-F | acttaagcttatgggtccaactggccctccagga | 2119 bp |
pcMINI-N-COL4A5-ECORI-R | tgcagaattctttgatctatgaaccaagat | ||
pcMINI-N mut left half fragment amplification | pcMINI-N-COL4A5-HindIII-F | acttaagcttatgggtccaactggccctccagga | 1473 bp |
COL4A5-mut-R | cgggtacctatttctacgaaataatcagta | ||
pcMINI-N mut right half fragment amplification | COL4A5-mut-F | tactgattatttcgtagaaataggtacccg | 662 bp |
pcMINI-N-COL4A5-ECORI-R | tgcagaattctttgatctatgaaccaagat |