Table 2 Primer sequences for quantitative real-time PCR validation.

From: Pro-neural transcription factors as cancer markers

Gene	Primer sense	Primer antisense
SDH	TGGGAACAAGAGGGCATCTG	CCACCACTGCATCAAATTCATG
GAPDH	GCAAATTCCATGGCACCGT	TCGCCCCACTTGATTTTGG
UBC	ATTTGGGTCGCGGTTCTTG	TGCCTTGACATTCTCGATGGT
TBP	GAATATAATCCCAAGCGGTTTG	ACTTCACATCACAGCTCCCC
Neuromedin/NT	GAGGAGCTTGTTGCAAGAAGGA	GCCCTGCTGTGACAGATTTTGT
Hes6	AGCTCCTGAACCATCTGCTC	GACTCAGTTCAGCCTCAGGG
Ascl1	GAGCAGGAGCTTCTCGACTTC	GATGCAGGTTGTGCGATCAC
Hes1	TGGAAATGACAGTGAAGCACCT	GTTCATGCACTCGCTGAAGC
NeuroD1	CGCTGGAGCCCTTCTTTG	GCGGACGGTTCGTGTTTG
DDC	AAGCACAGCCATCAGGATTCA	TGGACATGCTTGCGGATATAAG
Neurogenin 2	CGCATCAAGAAGACCCGTAG	GTGAGTGCCCAGATGTAGTTGTG

Primers for quantitative real time polymerase chain reaction using Sybrgreen chemistry were designed using Primer Express software (Applied Biosystems)

Back to article page