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Table 3 Top-ranking 45 antigens (sorted by quantitative and qualitative scores).

From: Whole genome identification of Mycobacterium tuberculosisvaccine candidates by comprehensive data mining and bioinformatic analyses

Group Rv # Gene (a) Length (aa) Annotation (a) Qual Total Quant Total
I Rv1738   94 hypothetical protein 9 14
  Rv2450c rpfE 172 probable resuscitation-promoting factor rpfE [transglycosylase] 9 14
  Rv2623 TB31.7 297 hypothetical protein TB31.7 [universal stress protein] 9 14
  Rv1009 rpfB 362 possible resuscitation-promoting factor rpfB [transglycosylase, C5 adhesion domain] 9 13
  Rv0867c rpfA 407 possible conserved trans-membrane protein [transglycosylase, rpfA] 9 12
  Rv2031c acr (α-crystallin) 144 heat-shock protein HspX (alpha-crystallin homolog) 14 kDa antigen Hsp16.3 8 14
  Rv1886c fbpB (Ag85B) 325 secreted antigen 85-B FBPB (85-B) (mycolyl-transferase 85B) 8 14
  Rv0288 esxH (TB10.4) 96 Low Mw protein antigen 7 esxH (10 kDa antigen) CFP-7, TB10.4) 8 13
  Rv2032 acg 331 conserved hypothetical protein Acg [nitroreductase] 8 13
  Rv2626c   143 hypothetical protein [CBS pair – binding/regulation, euk] 8 13
  Rv3873 PPE68 368 PPE family protein [PPE68, RD1 T/B immunogen] 8 13
  Rv2005c   295 hypothetical protein [USP-like] 8 12
  Rv3127   344 hypothetical protein [possible nitroreductase] 8 12
II Rv1733c   210 probable conserved trans-membrane protein 8 11
  Rv1996   317 hypothetical protein [USP] 8 10
  Rv2389c rpfD 154 probable resuscitation-promoting factor rpfD [transglycosylase] 8 10
  Rv0685 Tuf 396 elongation factor Tu [iron-regulated] 8 9
  Rv2628   120 hypothetical protein 8 9
  Rv1980c mpb64 228 immunogenic protein MPT64 7 13
  Rv3804c fbpA (Ag85A) 338 secreted antigen 85-A FBPA (85-A) (mycolyl-transferase 85A) 7 13
  Rv0079   273 hypothetical protein 7 11
  Rv3130c [tgs1] 463 hypothetical protein [diacylglycerol acyltransferase] 7 11
  Rv3131 [bfnB] 332 hypothetical protein [possible nitroreductase NfnB] 7 11
  Rv0824c desA1 389 probable acyl [-acyl-carrier-desaturase desA1] 7 10
  Rv1908c katG 740 catalase-peroxidase-peroxinitritase-T katG 7 10
  Rv1174c [sak5] 110 Low Mw T-cell antigen TB8.4 [secretion antigen SA5K] 7 9
  Rv1349 [irtB] 579 probable drugs transport ATP-binding protein ABC transporter [ATM1 ABC siderophore-iron transporter] 7 9
  Rv1813c   143 hypothetical protein 7 9
  Rv2006 otsB1 1327 probable trehalose-6-phosphate phosphatase OTSB1 7 9
  Rv2029c pfkB 339 possible phosphofructokinase (pfkB) 7 9
  Rv2627c   413 hypothetical protein 7 9
  Rv2780 ald 371 secreted L-alanine dehydrogenase ald (40 kDa antigen, TB43) 7 9
III Rv1884c rpfC 176 probable resuscitation-promoting factor rpfC [transglycosylase] 7 8
  Rv2620c   141 probable conserved transmembrane protein 7 8
  Rv2744c 35kd-Ag [pspA] 270 conserved 35 kDa Alanine-rich protein [phage-shock protein IM30] 7 8
  Rv3875 esxA 95 6 kDA early secretory antigenic target ESXA (ESAT-6) 6 11
  Rv1926c mpt63 159 immunogenic protein MPT63 (16 kDa immunoprotective extracellular protein) 6 10
  Rv2030c   681 hypothetical protein [putative esterase/transferase] 6 10
  Rv3132c devs 578 two component sensor histidine kinase DEVS 6 10
  Rv3347c PPE55 3157 PPE family protein (PE55) [8 copies pentapeptide repeats] 6 10
  Rv0467 icl 428 isocitrate lyase(icl) [AceA] 6 9
  Rv1130 [prpD] 526 hypothetical protein [2 methyl-citrate dehydratase] 6 9
  Rv1169c PE11 100 PE family protein (PE11) [triacyl glycerole lipase] 6 9
  Rv1793 esxN 94 putative ESAT-6-like protein ESXN (ESAT-6-like protein 5) 6 9
  Rv2629   374 hypothetical protein [peptide release factor erF1] 6 9
  1. The antigens are sorted by the qualitative score (Qual Total) and subsequently by the quantitative score (Quant Total) (see text). Group I includes all antigens with a qualitative score 8 and above, provided that the quantitative score is not lower than 12. The rest of the antigens having a qualitative score of 8 and those having a qualitative score of 7 and a quantitative score not lower than 9 were clustered into Group II. Group III included antigens with qualitative scores of 7 (and quantitative score of 8) and 6 (with a quantitative scores 9 and up). (a) The Gene name and annotation are based on the data deposited at the NCBI, [GenBank: AL123456]. In square brackets: updated gene name and/or annotation, resulting from the analyses conducted in this study.