mRNA levels of CAV1, ENO2, PKCα and DHFR upon siRNA treatment of MTX-resistant cells. Treatments with increasing amounts of each siRNA were performed in MTX-resistant HT29 cells. Forty-eight hours later, mRNA levels for each gene were determined and expressed as percentages of the untreated control (A, CAV1; B, ENO2; C, PKCα and D, DHFR). A non-related (NR) siRNA was used as negative control. Results are depicted taking into account the relative gene expression in the resistant cells (% of the sensitive cells). Values are the mean of three independent experiments ± SE.