Effect of combining the siRNA against CAV1 and the expression plasmid for E-cadherin. One hundred nanomolar siRNA against CAV1 and 1 μg of the expression plasmid for E-cadherin were transfected in both sensitive (A) and resistant (B) HT29 cells. MTX was added 48 hours after transfection and the MTT assay was used to determine cell viability. Results are expressed as percentages referred to untreated cells. Values are the mean of three independent experiments ± SE.