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Table 4 Chromosome 17 Fluorescence in situ Hybridization results of 40 NBTs obtained from MSKCC, displayed in relation to NBTs ploidy status

From: Differential expression of genes mapping to recurrently abnormal chromosomal regions characterize neuroblastic tumours with distinct ploidy status

Case Number

Ploidy

MYCN

FISH Chromosome 17

Disease Status

Survival Status

Cell % (# DNA probe signals: LSI 17p13.1: CEP 17)

1

near-3n

NA

23 (2:2), 44 (3:3), 33 (4:4)

NP

A

2

near-3n

NA

50 (2:2), 50 (3:3)

NP

A

3

near-3n

NA

16 (2:2), 41 (3:3), 43 (4:4)

NP

A

4

near-3n

NA

34 (2:2), 42 (3:3), 24 (4:4)

NP

A

5

near-3n

NA

33 (2:2), 50 (3:3), 17 (4:4)

P

A

6

near-3n

NA

25 (2:2), 60 (3:3), 15 (4:4)

NP

A

7

near-3n

NA

31 (2:2), 46 (3:3), 23 (4:4)

NP

A

8

near-3n

NA

35 (2:2), 52 (3:3), 13 (4:4)

NP

A

9

near-3n

NA

23 (2:2), 54 (3:3), 23 (4:4)

NP

A

10

near-3n

NA

13 (3:3), 66 (3:4), 21 (5:5)

NP

A

11

near-3n

NA

16 (2:2), 48 (3:3), 36 (4:4)

P

A

12

near-3n

NA

35 (2:2), 58 (3:3), 7 (4:4)

NP

A

13

near-3n

NA

46 (2:2), 24 (3:3), 30 (4:4)

NP

A

14

near-3n

NA

22 (3:3), 62 (4:4), 16 (4:5)

P

D

15

near-3n

NA

10 (2:2), 29 (3:3), 45 (4:4), 16 (5:5)

P

D

16

near-2n

NA

5 (1:1), 65 (2:2), 5 (1:2), 10 (3:3), 5 (2:3), 5 (4:4), 5 (3:4)

P

D

17

near-2n

NA

100 (2:2)

P

D

18

near-2n

NA

95 (2:2), 5 (3:3)

P

D

19

near-2n

NA

31 (CEP 2), 50 (CEP 3), 18 (CEP 4)

NP

A

20

near-2n

NA

25 (1:1), 75 (2:2)

P

D

21

near-2n

NA

100 (2:2)

P

D

22

near-2n

NA

n.e

P

D

23

near-2n

NA

10 (CEP 1), 40 (CEP 2), 38 (CEP 3), 12 (CEP 4)

P

A

24

near-2n

NA

80 (2:2), 10 (1:2), 5 (3:3), 5 (2:2)

P

A

25

near-2n

NA

100 (2:2)

P

D

26

near-2n

NA

25 (1:1), 75 (2:2)

P

D

27

near-2n

NA

100 (2:2)

P

D

28

near-2n

NA

5 (2:1), 74 (2:2), 5 (1:2), 5 (3:3), 6 (2:3), 5 (4:4)

P

D

29

near-2n

NA

10 (2:1), 70 (2:2), 15 (1:2), 5 (3:3)

P

D

30

near-2n

NA

n.e

P

D

31

near-2n

A

20 (2:2), 32 (3:3), 12 (4:3), 20 (4:4), 16 (3:4)

NP

A

32

near-2n

A

40 (1:1), 60 (2:2)

NP

A

33

near-2n

A

n.e

NP

A

34

near-2n

A

100 (2:2)

P

A

35

near-2n

A

35 (2:2); 5 (3:2), 20 (3:3), 5 (2:3), 30 (4:4), 5 (3:4)

P

D

36

near-2n

A

60 (2:2); 5 (3:2), 25 (3:3), 5 (4:3), 5 (4:4)

P

D

37

near-2n

A

10 (1:1), 90 (2:2)

P

D

38

near-4n

NA

29 (2:2), 6 (3:3), 8 (4:3), 37 (4:4), 20 (3:4)

NP

A

39

near-4n

NA

49 (2:2), 37 (3:3), 9 (2:3), 5 (3:4)

NP

A

40

near-4n

NA

6 (2:2), 20 (3:3), 5 (4:3), 46 (4:4), 18 (5:5), 5 (4:5)

P

A

  1. n.e = not evaluable results. MYCN amplification status: NA = not amplified, A = amplified. Disease status: NP = no disease progression, P = disease progression. Survival status: A = alive, D = dead. FISH: results are displayed as percentage of cells exhibiting the observed number of DNA probe signals, and exact number of signals for the DNA probes used: chromosome 1 (LSI 1p36 and LSI 1q25 DNA probes) and chromosome 17(LSI 17p13.1 and CEP 17 DNA probes). Array CGH: p and q = chromosome arms, cen. = centromeric; G = chromosome gain, L = chromosome loss. Q-PCR: gene copy number fold changes are determined by the ΔΔCT relative quantification method.