Skip to main content

Table 4 Chromosome 17 Fluorescence in situ Hybridization results of 40 NBTs obtained from MSKCC, displayed in relation to NBTs ploidy status

From: Differential expression of genes mapping to recurrently abnormal chromosomal regions characterize neuroblastic tumours with distinct ploidy status

Case Number Ploidy MYCN FISH Chromosome 17 Disease Status Survival Status
Cell % (# DNA probe signals: LSI 17p13.1: CEP 17)
1 near-3n NA 23 (2:2), 44 (3:3), 33 (4:4) NP A
2 near-3n NA 50 (2:2), 50 (3:3) NP A
3 near-3n NA 16 (2:2), 41 (3:3), 43 (4:4) NP A
4 near-3n NA 34 (2:2), 42 (3:3), 24 (4:4) NP A
5 near-3n NA 33 (2:2), 50 (3:3), 17 (4:4) P A
6 near-3n NA 25 (2:2), 60 (3:3), 15 (4:4) NP A
7 near-3n NA 31 (2:2), 46 (3:3), 23 (4:4) NP A
8 near-3n NA 35 (2:2), 52 (3:3), 13 (4:4) NP A
9 near-3n NA 23 (2:2), 54 (3:3), 23 (4:4) NP A
10 near-3n NA 13 (3:3), 66 (3:4), 21 (5:5) NP A
11 near-3n NA 16 (2:2), 48 (3:3), 36 (4:4) P A
12 near-3n NA 35 (2:2), 58 (3:3), 7 (4:4) NP A
13 near-3n NA 46 (2:2), 24 (3:3), 30 (4:4) NP A
14 near-3n NA 22 (3:3), 62 (4:4), 16 (4:5) P D
15 near-3n NA 10 (2:2), 29 (3:3), 45 (4:4), 16 (5:5) P D
16 near-2n NA 5 (1:1), 65 (2:2), 5 (1:2), 10 (3:3), 5 (2:3), 5 (4:4), 5 (3:4) P D
17 near-2n NA 100 (2:2) P D
18 near-2n NA 95 (2:2), 5 (3:3) P D
19 near-2n NA 31 (CEP 2), 50 (CEP 3), 18 (CEP 4) NP A
20 near-2n NA 25 (1:1), 75 (2:2) P D
21 near-2n NA 100 (2:2) P D
22 near-2n NA n.e P D
23 near-2n NA 10 (CEP 1), 40 (CEP 2), 38 (CEP 3), 12 (CEP 4) P A
24 near-2n NA 80 (2:2), 10 (1:2), 5 (3:3), 5 (2:2) P A
25 near-2n NA 100 (2:2) P D
26 near-2n NA 25 (1:1), 75 (2:2) P D
27 near-2n NA 100 (2:2) P D
28 near-2n NA 5 (2:1), 74 (2:2), 5 (1:2), 5 (3:3), 6 (2:3), 5 (4:4) P D
29 near-2n NA 10 (2:1), 70 (2:2), 15 (1:2), 5 (3:3) P D
30 near-2n NA n.e P D
31 near-2n A 20 (2:2), 32 (3:3), 12 (4:3), 20 (4:4), 16 (3:4) NP A
32 near-2n A 40 (1:1), 60 (2:2) NP A
33 near-2n A n.e NP A
34 near-2n A 100 (2:2) P A
35 near-2n A 35 (2:2); 5 (3:2), 20 (3:3), 5 (2:3), 30 (4:4), 5 (3:4) P D
36 near-2n A 60 (2:2); 5 (3:2), 25 (3:3), 5 (4:3), 5 (4:4) P D
37 near-2n A 10 (1:1), 90 (2:2) P D
38 near-4n NA 29 (2:2), 6 (3:3), 8 (4:3), 37 (4:4), 20 (3:4) NP A
39 near-4n NA 49 (2:2), 37 (3:3), 9 (2:3), 5 (3:4) NP A
40 near-4n NA 6 (2:2), 20 (3:3), 5 (4:3), 46 (4:4), 18 (5:5), 5 (4:5) P A
  1. n.e = not evaluable results. MYCN amplification status: NA = not amplified, A = amplified. Disease status: NP = no disease progression, P = disease progression. Survival status: A = alive, D = dead. FISH: results are displayed as percentage of cells exhibiting the observed number of DNA probe signals, and exact number of signals for the DNA probes used: chromosome 1 (LSI 1p36 and LSI 1q25 DNA probes) and chromosome 17(LSI 17p13.1 and CEP 17 DNA probes). Array CGH: p and q = chromosome arms, cen. = centromeric; G = chromosome gain, L = chromosome loss. Q-PCR: gene copy number fold changes are determined by the ΔΔCT relative quantification method.