Figure 2

Heatmap visualization of gene expression data from PKA subunit transfections. 2A. Combined proliferation/differentiation genes (1933/1660 genes, respectively: Supplementary Data 4) computationally derived from a total of 11,048 genes detected (2B, and Supplementary Data 1). All samples in triplicate (biological replicates), gene expression normalized by rows as well as by individual samples to enhance visualization. 2C. Heatmap of a subset of GSMA enrichment scores using gene lists of pathway and gene signatures from the Broad Institute (MSigDB c2: Curated Gene Sets) (see Supplementary Data 3 for all GSMA scores). Red indicates positive enrichment (median gene expression of all genes in list > 0), green indicates negative enrichment (median gene expression of all genes in list < 0). Datasets tested include z ratios and log fold changes generated for PKA subunits samples using both Affymetrix U133 Plus 2.0 arrays as well as Illumina BeadArrays.