GSEA gene set analysis of PKA subunit regulated genes versus human myotube differentiation data. The top 250 RIα, RIIβ, and myotube significantly up- and down-regulated genes are graphically displayed as indicated across a rank ordered distribution of all myotube versus myoblast changes in gene expression. The red curves report the values of a modified Kolmogorov-Smirnoff cumulative statistic  across the distribution of all changes in gene expression (of the myotube dataset). The peak of the curve (either above or below the midpoint of zero) becomes the maximum enrichment score (ES) which is normalized by gene list size to generate a normalized enrichment score (NES) and tested for significance by permutation analysis (NOM p-val, as reported in Table 1). Myotube up- or down-regulated genelists were used as positive controls. RIIβ and RIα up- and down-regulated genes are shown to cross correlated with myotube changes in gene expression (red circles indicate areas of enrichment).