Protein expression profiles of (A) BCL2A (C) BBC3 and (E) active CASP3. Protein expression kinetics supports the transcriptional patterns of selected genes demonstrated by microarray analysis. CD3+ T cells were selected, stimulated (with anti-CD3/anti-CD28 antibodies), cultured and harvested at the indicated timepoints of culture to analyze the protein expression by flow cytometric assays. For BCL2A and BBC3, data from two independent experiments, E4 and E5, are shown; for CASP3, data from three independent experiments, E1, E4 and E5, are shown. (B), (D) and (F) demonstrated the transcriptional patterns of BCL2A1, BBC3 and CASP3 in all three populations (CD3+, CD4+ and CD8+).