Significantly regulated genes in NF-κB signalling pathway. (A) Expression profiles of genes involved in NF-κB signalling pathway. Color denotes degree of differential expression compared to 0 hour (saturated red = 3-fold up-regulation, saturated green = 3-fold down-regulation, black = unchanged, gray = no data available). Expression data shown are averages from three independent biological experiments for each T-cell population. The median ratio, along with the maximum (for up-regulated genes) or minimum (for down-regulated genes) ratio of stimulated T cells at each timepoint (with respect to the expression of 0 hour) is provided (a negative value represents down-regulation). Genes, whose transcription can induce the activation of the NF-κB complex, identified in a large scale screening study  were shown in blue. (B) Protein expression profile of phosphorylated p65 of NF-κB complex. CD3+ T cells were selected, stimulated (with anti-CD3/anti-CD28 antibodies), cultured and harvested at the indicated timepoints of culture to analyze the protein expression by flow cytometric assays. Data from two independent experiments, E4 and E5, are shown. (C) The transcriptional pattern of RELA in all three populations (CD3+, CD4+ and CD8+).