Figure 2

LDL binding and uptake characteristics of monocytes of FH patients and healthy donors. A-E: Binding and uptake of oxidized LDL (homozygous FH (black triangles) n = 4, heterozygous FH (gray triangles) n = 4 and healthy controls (open triangles) n = 4) and native LDL (homozygous FH (black circles) n = 4, heterozygous FH (gray circles), healthy controls (open circles) n = 4) by monocytes. A: Binding of AF488 labelled oxLDL and B: nLDL for 1 h at 4°C. C: Dose dependent uptake of AF488 labelled oxLDL and D: nLDL for 1 h at 37°C. E: Time dependent uptake of 100 μg/ml AF488 labelled oxLDL and E: nLDL for up to 4 h; please note that due to separate lipoprotein preparations for each experiment fluorescence intensities cannot be compared between subpanels A-E; comparison is only possible within a subpanel; experiments were done in duplicate; histogram bars indicate the mean ± SD (* p < 0.05, ** p < 0.01).