Figure 4

Increased CD36 and CD91/Lrp1 levels on the surface of monocytes from FH patients and their role in LDL uptake. A: Representative measurements by flow cytometry of the cell surface expression of CD36 and CD91/Lrp1 on monocytes of a control (no filling), of a heterozygous FH patient (gray filling) and of a homozygous FH patient (black filling). One representative measurement of an antibody isotype control is illustrated as a black dotted line. The y-axis shows the percentage of maximum events. B: Western Blot analysis of CD91/Lrp1 of four homozygous FH patients and four controls. β-actin was used as loading control. C: Monocytes of homozygous FH patients (black filled bars), heterozygous FH patients (grey filled bars) and control individuals (open bars) were incubated with CD36-blocking antibody FA6-152, control murine IgG or left untreated for 0,5 h at 37°C; after incubation cells were incubated with 100 μg/ml AF647 labelled oxLDL for 4 h at 37°C. (homozygous FH n = 5,, heterozygous FH patients n = 4, healthy controls n = 5) D: Monocytes of homozygous FH patients (black filled bars), heterozygous FH patients (grey filled bars) and control individuals (open bars) were incubated with CD91/Lrp1-blocking antibody, control rabbit IgG or left untreated for 0,5 h at 37°C; after incubation cells were incubated with 100 μg/ml AF647 labelled nLDL for 4 h at 37°C. Experiments in C and D were done in duplicate. Histogram bars indicate the mean ± SD (** p < 0.01, *** p < 0.001).