A , expression of miR-34b/34c/449 is enriched in normal lung, fallopian tube, and testis. CT higher than 35 is generally considered as less reliable, and equals to around 5 copies of miRNA. Dashed line: around 20 copies of miRNA. Expression of these 3 miRNAs is much higher than this line in lung, fallopian tube, and testis than in the rest of tissues examined. B, expression of miR-34b/34c/449 is reduced in the 9 lung cancer cell lines of the NCI-60 panel compared to normal lung, represented by ΔCT (tumor cell line – normal lung). C, expression of miR-34b/34c is reduced in both human and mouse primary lung cancer tissues compared to normal lung. The log2-based background fluorescence intensity is between 5 and 6, so tumor lung and non-lung normal human tissues have essentially no miR-34b expression and some minimally detectable miR-34c, consistent with the TaqMan®-based results in the panel A. D, expression of the 17 "core" genes in specimens from the Database 1; blue bar, genes with higher expression in SCC; red bar, genes with higher expression in AD; *, two genes with higher expression in normal lung; scale bar represents fold change while gray in the heat map indicates missing data.