Figure 4

Responses of genes regulated by p53 and NFκB. Ingenuity Pathways Analysis was used to extract nodes having direct regulatory interactions with p53 or NFκB from the set of genes significantly responding to radiation. Expression levels are overlaid on the nodes so that up-regulated genes are displayed in red and down-regulated genes in green. The scale bar shows the range of fold-changes. Panel a) p53 regulated genes are overlaid with relative expression levels in irradiated (left) or bystander (right) cells 4 hours after treatment. Just under half the genes changing in irradiated cells respond in bystanders. Panel b) NFκB regulated genes overlaid with relative expression in irradiated (left) and bystander (right). The responses are nearly identical at the 4-hour time point.