Table 2 Real time PCR sequences used in individual assays
Gene name | Primer/probe | Primer sequence |
|---|---|---|
CDKN1A a | forward | 5' CTG GAG ACT CTC AGG GTC GAA |
| Â | reverse | 5' CGG CGT TTG GAG TGG TAG AA |
| Â | probe | 5' TCA TGC TGG TCT GCC GCC GT |
IL8 | forward | 5' AAGACATACTCCAAACCTTTCCA |
| Â | reverse | 5' CCAGACAGAGCTCTCTTCCA |
| Â | probe | 5' TGGACCACACTGCGCCAACA |
PTGS2 | forward | 5' AAGACATACTCCAAACCTTTCCA |
| Â | reverse | 5' CCAGACAGAGCTCTCTTCCA |
| Â | probe | TGGACCACACTGCGCCAACA |
BCL2A1 | forward | 5' TTGGATATATTTACAGGCTGGCT |
| Â | reverse | 5'GACCTGATCCAGGTTGTGG |
| Â | probe | 5' CAGGACTATCTGCAGTGCGTCCTACAG |