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Table 4 Comparison of relative gene expression by microarray and qRT-PCR

From: Global gene expression analyses of bystander and alpha particle irradiated normal human lung fibroblasts: Synchronous and differential responses

  

Irradiated

Bystander

Gene

Description

Arraya

qRT-PCRb

Array

qRT-PCR

CDKN1A

cyclin dependent kinase inhibitor, p21

5.7

6.7

1.3

1.4

FDXR

Ferredoxin reductase

2.2

1.9

1.1

0.8

MDM2

Human mouse double minute, p53 binding

2.8

3.7

1.3

1.7

GADD45A

growth arrest & DNA damage inducible

3.5

7.2

2.1

3.5

GDNF

Glial cell line-derived neurotrophic factor precursor

5.1

8.8

1.7

3.6

DDB2

damage-specific DNA binding protein 2

2.2

1.9

1.1

0.9

ATF3

activating transcription factor 3

1.5

2.2

0.9

1.1

FAS

TNF receptor superfamily, member 6

2.3

3.1

1.3

1.5

GDF15

growth differentiation factor 15

8.6

9.6

3.6

3.2

IL6

interleukin 6

11.2

12.7

17.1

14.7

IL8

interleukin 8

12.3

20.1

17.9

27.4

IL1B

interleukin 1 beta

3.6

3.4

4.4

3.9

IL1A

interleukin 1 alpha

4

2.7

4.3

3.2

IL33

interleukin 33

6

7.8

5.4

6.7

PTGS2

cyclooxygenase-2

6

6.2

6.4

6.8

CXCL2

Chemokine CXC ligand 2

4.1

5.9

5.2

7

CXCL3

Chemokine CXC ligand 3

6

6.9

8.9

7.9

CXCL5

Chemokine CXC ligand 5

4.8

4.7

6

5.5

BCL2A1

BCL2 related protein1

5

5

7.4

5.8

LIF

Leukemia inhibitory factor

3.1

3.5

3.7

3.7

FGF2

fibroblast growth factor 2

3.2

3.9

2.7

3.5

POU5F1

POU domain, class 5, transcription factor 1

3.2

2.1

1.5

1.5

ICAM1

intercellular adhesion molecule 1 (CD54)

2.3

2.5

2.6

2.7

MMP1

matrix metallopeptidase 1

2.8

3.6

3.5

3.4

MMP3

matrix metallopeptidase 3

5.2

5.5

4.2

4.7

MT1X

metallothionein 1X

4.6

13.1

4.7

17.4

MT1H

metallothionein 1H

4.6

2.8

4.7

2.5

TNFAIP3

tumor necrosis factor, alpha-induced prot. 3

2.7

3

3.4

3.4

SOD2

superoxide dismutase 2

2

1.9

2.3

2.6

SERPINB2

Plasminogen activator inhibitor 2 precursor

11.4

13.9

12.6

13.3

LAMB3

laminin, beta 3

2.6

2.4

3.1

2.8

KYNU

kynureninase

3.1

3.3

3.6

3.9

GJA1

gap junction protein, alpha 1, 43 kDa (connexin 43)

1.8

2.3

1.6

1.6

GJB2

gap junction protein, beta 2, 26 kDa (connexin 26)

1.9

1.9

2.4

1.6

DUSP2

dual specificity phosphatase 2

4.5

8.8

7.9

10.4

CARD9

caspase recruitment domain fam., member 9

0.7

0.6

0.7

0.5

DKK1

dickkopf homolog 1

3.2

3.1

2.4

2.7

  1. a Mean of treated to control ratios measured by microarray in 4 independent experiments.
  2. b Mean of qRT-PCR measurements of the same RNA used in microarray analyses.
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BMC Medical Genomics

ISSN: 1755-8794

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