Table 2 Candidate cardiac reference genes ranked in order of abundance
From: Genomic selection of reference genes for real-time PCR in human myocardium
UniGene accession | Gene symbol | Gene name | Gene ontology biological process | Location | HF patients* (copy #/μg total RNA) | Controls* (copy #/μg total RNA) | p-value (HF vs controls) |
|---|---|---|---|---|---|---|---|
Hs.479728 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | glucose metabolism, glycolysis, translational initiation, cell communication, | 12p13 | 1.13 × 109 (0.91 – 1.40 × 109) | 1.65 × 109 (1.33 – 2.05 × 109) | 0.015 |
Hs.515329 | RPL22 | ribosomal protein L22 | protein biosynthesis | 1p36 | 1.48 × 107 (1.13 – 1.95 × 107) | 2.80 × 107 (2.13 – 3.68 × 107) | 0.002 |
Hs.374596 | TPT1 | tumor protein, translationally-controlled 1 | calcium and microtubule-binding | 13q12 | 1.00 × 107 (0.60 – 1.69 × 107) | 1.93 × 107 (1.15–3.26 × 107) | 0.080 |
Hs.446628 | RPS4X | ribosomal protein S4, X-linked | regulation of cell cycle, protein biosynthesis, development, cell proliferation | Xq13 | 0.89 × 107 (0.62 – 1.27 × 107) | 1.99 × 107 (1.39 – 2.86 × 107) | 0.003 |
Hs.523185 | RPL13A | ribosomal protein L13a | protein biosynthesis | 19q13 | 2.33 × 106 (1.39–3.90 × 106) | 6.24 × 106 (3.73 – 10.45 × 106) | 0.009 |
Hs.419463 | RPL23A | ribosomal protein L23a | protein biosynthesis | 17q11 | 2.44 × 106 (1.78 – 3.34 × 106) | 5.51 × 106 (4.02 – 7.56 × 106) | 0.001 |
Hs.490287 | EEF1A1 | Eukaryotic elongation factor 1A1 | translational elongation | 6q14 | 2.44 × 106 (1.42 – 4.17 × 106) | 4.47 × 106 2.61–7.65 × 106 | 0.116 |
Hs.112553 | RPL41 | ribosomal protein L41 | protein biosynthesis | 12q13 | 1.78 × 106 (1.08 – 2.93 × 106) | 4.70 × 106 (2.86 – 7.73 × 106) | 0.008 |
Hs.355643 | RNPS1 | RNA binding protein S1 | transcription, RNA splicing | 16p13 | 6.19 × 105 (4.04 – 9.48 × 105) | 11.00 × 105 (7.19 – 16.85 × 105) | 0.061 |
Hs.533732 | SRP14 | signal recognition particle 14 kDa | protein targeting | 15q22 | 4.03 × 105 (2.30 – 7.08 × 105) | 10.76 × 105 (6.14 – 18.90 × 105) | 0.017 |