Table 4 Normalization of NPPB using fewer than three reference genes
From: Genomic selection of reference genes for real-time PCR in human myocardium
Genes used for normalization | Correlation with normalization factors using TPT1, EEF1A1 & RNPS1 | Normalized NPPB levels (geometric mean and 95% CI, copy #/μg total RNA) | |||
|---|---|---|---|---|---|
| Â | PCC | p-value for correlation | HF patients | Controls | p-value (HF vs controls) |
TPT1, EEF1A1 & RNPS1 | 1.000 | - | 11.3 × 106 (5.3 – 24.0 × 106) | 3.3 × 106 (1.5 – 6.9 × 106) | 0.023 |
TPT1 & EEF1A1 | 0.989 | <0.001 | 11.2 × 106 (5.3 – 23.9 × 106) | 3.3 × 106 (1.5 – 7.0 × 106) | 0.024 |
GAPDH | -0.085 | 0.535 | 77.5 × 106 (35.6 – 168.9 × 106) | 18.5 × 106 (8.5 – 40.4 × 106) | 0.012 |
Raw data | - | - | 14.1 × 106 (6.6 – 30.4 × 106) | 4.9 × 106 (2.3 – 10.6 × 106) | 0.058 |