Expression profiling with RNA from formalin-fixed, paraffin-embedded material

BMC Medical Genomics

Table 1 QPCR assays. QPCR assays (Assays on Demand) were from Applied Biosystems (Palo Alto, CA). Reverse primers from each assay were used for the synthesis of gene-specific cDNAs. They were provided separately by Applied Biosystems. Three assays (IGBP5_short, IGBP5_medium, IGBP5_long) were designed manually.

AoD	Assay	Acc_Nr	AmpliconSize	Module
Hs00608023_m1	BCL2	NM_000633	81	Estrogen
Hs00221277_m1	CEGP1	NM_020974	64	Estrogen
Hs00174860_m1	ESR1	NM_000125	62	Estrogen
Hs00172183_m1	PGR	NM_000926	118	Estrogen
Hs00180450_m1	GRB7	NM_005310	70	Her2
Hs01001598_g1	HER2	NM_004448	55	Her2
Hs00952036_m1	CTSL2	NM_001333	72	Invasion
Hs00171829_m1	STMY3	NM_005940	66	Invasion
Hs01030097_m1	CCNB1	NM_031966	66	Proliferation
Hs01032443_m1	MKI67	NM_002417	66	Proliferation
Hs00231158_m1	MYBL2	NM_002466	81	Proliferation
Hs00269212_m1	STK15	NM_003600	85	Proliferation
Hs00153353_m1	SURV	NM_001168	93	Proliferation
Hs99999903_m1	ACTB	NM_001101	171	Reference
Hs00266705_g1	GAPDH	NM_002046	74	Reference
Hs99999908_m1	GUSB	NM_000181	81	Reference
Hs99999902_m1	RPLP0	NM_001002	105	Reference
Hs00174609_m1	TFRC	NM_003234	79	Reference
Hs00430290_m1	UBB	NM_018955	120	Reference
Hs01630490_s1	RPL7A	BX641050	84	Reference
Hs00817975_g1	RPS11	NM_001015	168	Reference
Hs01922548_s1	RPS23	NM_001025	90	Reference
Hs00185390_m1	BAG1	NM_004323	58
Hs00154355_m1	CD68	NM_001251	68
Hs01383449_s1	GSTM1	AY532925	65
(own design)	IGBP5_short	NM_000599	60	Test
(own design)	IGBP5_medium	NM_000599	109	Test
(own design)	IGBP5_long	NM_000599	147	Test

ISSN: 1755-8794