Analisis of methylation status of 3.3-kb and Sat2 repeats in HPV-positive cervical tumors by Southern blot hybridization. Nc, normal cervix from non-cervical-cancer patients; T, tumor; N, tumor-adjacent normal tissue. Lines to the left of the panel are molecular mass markers (from top to bottom, 24.3, 9.4, 6.6, 4.4, 2.3, 2.0, and 0.5 kb). (A) Hybridization with the 3.3-kb repeat-specific probe, NaeI digests of DNAs. Numbers under the panels indicate the percentage of the high-molecular-weight hybridization signal (>4.4 kb) to the total hybridization signal for each track. (B) Hybridization with the Sat2-specfic oligonucleotide, BstBI digests of DNAs. (C) Hypermethylation of 3.3-kb repeats in tumors. (D) Hypomethylation of Sat2 in tumors. The asterisk and triangle indicate samples with hypermethylation and hypomethylation of 3.3-kb repeats, respectively; the circle – unchanged methylation status of 3.3-kb repeats.