Figure 3

RT-PCR assay and western blot analysis. Detection of over-expression of defensin-α1 in CFS patients compared to normal healthy controls. A) 25 μl of each PCR product was electrophoresed through a 2.5% agarose gel, ethidium bromide stained and UV visualised. Lanes: L: DNA size ladder; 1–3: Healthy controls; 4–6: Patients with CFS. B) Western blot analysis. Each lane contained 2 × 10⁵ PBMC and equivalent protein loading of all samples was confirmed by coomassie blue staining. Left-hand side of blot: 10 CFS patients; right-hand side of blot: 10 healthy controls.