Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

Table 3 qRT-PCR on multiple sclerosis patients and normal controls

Gene Name	Ct-avg	SD	CV	n
TRAP1	25.72	0.62	0.024	40
B2M	17.93	0.78	0.044	40
DECR1	25.72	0.79	0.031	40
PPIB	24.67	0.82	0.033	40
FPGS	27.88	0.84	0.030	40
HMBS	29.02	0.94	0.032	40
FARP1	35.79	0.98	0.027	35
MAPRE2	27.39	1.01	0.037	40
PEX16	27.95	1.09	0.039	40
GINS2	32.42	1.10	0.034	40
ACTB	36.66	1.11	0.030	23
GAPDH	22.07	1.32	0.060	40
CRY2	29.73	1.37	0.046	40
CSNK1G2	28.64	1.49	0.052	40
A4GALT	34.92	1.69	0.048	32

Average raw Cycle threshold values (Ct-avg), standard deviation (SD), coefficient of variability (CV) and sample size (n) for the qRT-PCR analyses of RNA samples from 20 patients with multiple sclerosis compared to 20 age and gender matched control subjects. Because those samples that had a SD > 1.41 for the duplicates were excluded from the analysis, the sample size is less than 40 for some of the genes.

ISSN: 1755-8794