Figure 3
From: Gene expression profile analysis of human hepatocellular carcinoma using SAGE and LongSAGE
Real-time quantitative RT-PCR validation of SAGE data. Expression level of candidate genes identified to be up-regulated in HCC or HepG2 by SAGE data was examined in 20 pairs of HCC and corresponding nontumorous liver tissues by quantitative RT-PCR. Increased expression level in HCC was observed in CCL20, COL4A1, XAGE-1, PEG10, SGCE, S100P, TM4SF1 and ZNF83 genes (p < 0.05). Red bars represent expression level of HCC tissues, blue bars represent nontumorous liver tissues, and horizontal bars represent SD values.