MicroRNA-125a is over-expressed in insulin target tissues in a spontaneous rat model of Type 2 Diabetes

BMC Medical Genomics

Table 1 Overlap between predicted miR-125a target genes and genes significantly altered in GK compared to BN rats in adipose tissue and in liver.

	miRanda		PicTar		TargetScan		>1 Algorithm
Predicted miR-125a target genes	975		350		165		152
Number genes tested	536		231		94		114
	Overlap	FDR	Overlap	FDR	Overlap	FDR	Overlap	FDR
Liver up-regulated (95 genes)	5	0.074	1	0.63	1	0.33	1	0.40
Liver down-regulated (138 genes)	5	0.248	2	0.43	0	1.00	0	1.00
Adipose up-regulated (477 genes)	21	0.006	5	0.57	1	0.87	3	0.46
Adipose down-regulated (598 genes)	22	0.036	7	0.44	1	0.92	0	1.00

The number of predicted miR-125a target genes found among the significantly up- and down-regulated genes in each tissue is shown for each algorithm and for the subset of targets predicted by more than one algorithm. In each case, the significance of the overlap (estimated false discovery rate) is calculated as the proportion of 10,000 random sets of non-miR-125 target genes of the same size showing equal or greater overlap.

ISSN: 1755-8794