Figure 6

Clorgyline downregulated EZH2 and upregulated its target genes in E-CA-88 cells. (A) Expression of EZH2 was decreased and its target, ADRB2, was increased at 24 hr after clorgyline treatment as determined by qRT-PCR. Expression of 3 of 4 PcG signature genes was upregulated in clorgyline-treated cells compared to control as determined by qRT-PCR. (B) Expression of PcG signature genes was upregulated after clorgyline treatment at 6 and 24 hr as determined by microarray. Asterisks indicate genes upregulated at both 6 and 24 hr.