Signal distribution by plate and input RNA. Left panel: Pre-normalization box and whisker plots depicting distribution of probe intensities for technical replicates on SAMs 1, 2, and 3. The vertical axis is log base 2 intensity and the horizontal axis is SAM well ID. The bottom and top of the box represent the 25th and 75th percentiles of the probe expression values. The horizontal bar inside the box represents the median. The whiskers extend to either 1.5 times the interquartile range (75th percentile minus the 25th percentile) or the most extreme point, whichever is shorter. Points beyond the whiskers indicate outliers. No global shift in distribution is seen between plates. Right panel: Pre-normalization box and whisker plots depicting distribution of probe intensities at various amounts of starting material. The vertical axis corresponds to a log base 2 intensity, while the horizontal axis corresponds to the ng of starting material.