Figure 5

Signal distribution by plate and input RNA. Left panel: Pre-normalization box and whisker plots depicting distribution of probe intensities for technical replicates on SAMs 1, 2, and 3. The vertical axis is log base 2 intensity and the horizontal axis is SAM well ID. The bottom and top of the box represent the 25^th and 75^th percentiles of the probe expression values. The horizontal bar inside the box represents the median. The whiskers extend to either 1.5 times the interquartile range (75^th percentile minus the 25^th percentile) or the most extreme point, whichever is shorter. Points beyond the whiskers indicate outliers. No global shift in distribution is seen between plates. Right panel: Pre-normalization box and whisker plots depicting distribution of probe intensities at various amounts of starting material. The vertical axis corresponds to a log base 2 intensity, while the horizontal axis corresponds to the ng of starting material.