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Table 4 qPCR results comparing methods of template amplification

From: Examining smoking-induced differential gene expression changes in buccal mucosa

Template

Itga5b 3'

Itga5b WT

Tmem8 3'

Tmem8 WT

Ankrd28 3'

Ankrd28 WT

Buccal 4

31.25

39.15

20.38

24.96

20.68

31.8

Buccal 5

35.81

No Ct

20.36

25.31

20.92

31.36

Blood 4

21.43

23.96

21.87

30.68

20.33

29.43

Blood 5

20.56

23.9

21.11

30.23

20.22

29.82

Control

21.18

23.8

21.63

28.4

20.23

29.47

  1. All amplifications were performed using either the Ovation V2 (3') or WT pico Nugen Kits, see Materials and Methods. Control RNA is from a pooled blood sample, see Materials and Methods.
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BMC Medical Genomics

ISSN: 1755-8794

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