Figure 2

C5L2 expression is increased in HAT group while similar immune receptors are unaltered. (A) C5L2 gene expression in SC and OM adipose tissue from control non-obese (NO), LAT, and HAT subjects as assessed by ΔΔ real time RT-PCR for C5L2 relative to the housekeeping gene B2M. 2-way ANOVA indicates significant differences (p < 0.0001) with a difference between groups (p < 0.0001) but no significant difference between tissues (pNS). Post-hoc test indicates that HAT was significantly increased vs LAT in SC (p < 0.0.01) and OM (p < 0.001), and HAT was significantly increased vs NO (non-obese) in both SC (p < 0.05) and OM ((p < 0.001) where * p < 0.05, ** p < 0.01 and *** p < 0.001 for HAT vs LAT and + p < 0.05, ++ p < 0.01 and +++ p < 0.001 for HAT vs NO. There were no significant differences between LAT and NO. (B) Gene expression of immune receptor genes related to C5L2 in SC adipose tissue from LAT (hatched bars) and HAT (solid bars) as assessed by microarray where LAT expression is set at 100%. No significant differences between the groups were found for any of the related receptors. Data is expressed as means ± SEM.