Table 2 Sequences of primers for real-time RT-PCR assays.
| Â | sense | antisense |
|---|---|---|
Genes selected for validation in CD34+ cells | ||
MY5OC | 5'CGTGGCAGAAGAGGCATACA3' | 5'AGCGAGCCGACACTGTCTTT3' |
GCDH_ncRNA | 5'aaggagctttgggtttttgt3' | 5'cgccttagtgacagtctccag3' |
HCK | 5'GAGTTCATGGCCAAAGGAAG3' | 5'GGAGGTCTCGGTGGATGTAG3' |
PPIF_ncRNA | 5'atcgagctttgggggtagat3' | 5'agcaacagtgtagcgcaatg3' |
NR4A2 | 5'ACTCCAACCCGGCTATGAC3' | 5'CATGGAGCCAGTCAGGAGAT3' |
NR4A2_ncRNA | 5'cttccgggtgtctgagaaag3' | 5'tttgcatgtgctaggagctg3' |
NR4A3 | 5'TCCAGATACTGTCCCACTGACC3' | 5'TCTGGATACATCAATGGAGGCT3' |
NR4A3_ncRNA | 5'ccgttaagcacctggttttc3' | 5'aggcgctgagaattattgga3' |
Genes selected for validation in stromal cells | ||
SPINT2 | 5'TCTGTTTCTCTGGGAGGTAGGA3' | 5'CGATCAGCGAGGAAACAACT3' |
HLA-E | 5'ATCGTGGGCATCATTGCT3' | 5'GAGTAGCTCCCTCCTTTTCCA3' |
SEMA3A | 5'AGGGACCGAAAACAACGTC3' | 5'CGTGGGTCCTCCTGTTTCTA3' |
TBCD_ncRNA | 5'TCTTGCCTGGTGAGTGTGAG3' | 5'AAGGTGTTGGAAGGGAGGAG3' |
SOLH_ncRNA | 5'AGTCTCCAGAGTGGCATGTG3' | 5'AAAGGTAGGCAGGGGAGGTA3' |
Endogenous gene | ||
HPRT | 5'TCCAGCAGGTCAGCAAAGAA3' | 5'GAACGTCTTGCTCGAGATGT3' |